Article

 

The Instrumentation of a Microfluidic Analyzer Enabling the Characterization of the Specific Membrane Capacitance, Cytoplasm Conductivity, and Instantaneous Young's Modulus of Single Cells. Öffentlichkeit Deposited

https://scholar.colorado.edu/concern/articles/8s45q948m
Abstract
  • This paper presents the instrumentation of a microfluidic analyzer enabling the characterization of single-cell biophysical properties, which includes seven key components: a microfluidic module, a pressure module, an imaging module, an impedance module, two LabVIEW platforms for instrument operation and raw data processing, respectively, and a Python code for data translation. Under the control of the LabVIEW platform for instrument operation, the pressure module flushes single cells into the microfluidic module with raw biophysical parameters sampled by the imaging and impedance modules and processed by the LabVIEW platform for raw data processing, which were further translated into intrinsic cellular biophysical parameters using the code developed in Python. Based on this system, specific membrane capacitance, cytoplasm conductivity, and instantaneous Young's modulus of three cell types were quantified as 2.76 ± 0.57 μF/cm², 1.00 ± 0.14 S/m, and 3.79 ± 1.11 kPa for A549 cells (
Creator
Date Issued
  • 2017-06-19
Academic Affiliation
Journal Title
Journal Issue/Number
  • 6
Journal Volume
  • 18
Subject
Zuletzt geändert
  • 2019-12-06
Identifier
  • PubMed ID: 28629175
Resource Type
Urheberrechts-Erklärung
DOI
ISSN
  • 1422-0067
Language
License

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