Undergraduate Honors Thesis
Packaging of viral microRNAs in gammaherpesvirus 68 Público Deposited
- Abstract
MicroRNAs are a characteristic of many herpesvirus genomes. These short, non-coding RNAs are capable of post-transcriptional regulation of target mRNAs. Recently, packaged miRNAs have been observed in purified virions of EBV and KSHV, gammaherpesviruses pathogenic in humans. Gammaherpesvirus 68 serves as a small-animal model of gammaherpesvirus pathogenesis. It encodes eight RNA pol III transcripts known as TMERs, which include viral tRNA-like genes adjacent to one or two miRNA genes. Here, we focused on the miRNAs of TMERs 1 and 5. We detected viral miRNAs and their precursors in purified virions of wild-type gammaherpesvirus 68, as well as two mutant viruses that do not produce TMERs. This suggests viral miRNA packaging is a preferred, organized process in virion maturation. We have also established that these miRNAs are biologically active upon infection.
- Creator
- Date Awarded
- 2013-04-04
- Academic Affiliation
- Advisor
- Granting Institution
- Subject
- Última modificación
- 2019-12-18
- Resource Type
- Declaración de derechos
- Language
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packagingOfViralMicrornasInGammaherpesvirus68.pdf | 2019-11-19 | Público | Descargar |