Article

 

Nucleic acid-binding specificity of human FUS protein. 公开 Deposited

https://scholar.colorado.edu/concern/articles/s1784m45f
Abstract
  • FUS, a nuclear RNA-binding protein, plays multiple roles in RNA processing. Five specific FUS-binding RNA sequence/structure motifs have been proposed, but their affinities for FUS have not been directly compared. Here we find that human FUS binds all these sequences with Kd (app) values spanning a 10-fold range. Furthermore, some RNAs that do not contain any of these motifs bind FUS with similar affinity. FUS binds RNA in a length-dependent manner, consistent with a substantial non-specific component to binding. Finally, investigation of FUS binding to different nucleic acids shows that it binds single-stranded DNA with three-fold lower affinity than ssRNA of the same length and sequence, while binding to double-stranded nucleic acids is weaker. We conclude that FUS has quite general nucleic acid-binding activity, with the various proposed RNA motifs being neither necessary for FUS binding nor sufficient to explain its diverse binding partners.

Creator
Date Issued
  • 2015-09-03
Academic Affiliation
Journal Title
Journal Issue/Number
  • 15
Journal Volume
  • 43
File Extent
  • 7535-7543
Subject
Publisher
最新修改
  • 2020-01-09
Identifier
  • PubMed ID: 26150427
Resource Type
权利声明
DOI
ISSN
  • 1362-4962
Language

关联

属于 Collection:

单件