Article

 

DNA-mediated association of two histone-bound complexes of yeast Chromatin Assembly Factor-1 (CAF-1) drives tetrasome assembly in the wake of DNA replication. Public Deposited

https://scholar.colorado.edu/concern/articles/7d278t601
Abstract
  • Nucleosome assembly in the wake of DNA replication is a key process that regulates cell identity and survival. Chromatin assembly factor 1 (CAF-1) is a H3-H4 histone chaperone that associates with the replisome and orchestrates chromatin assembly following DNA synthesis. Little is known about the mechanism and structure of this key complex. Here we investigate the CAF-1•H3-H4 binding mode and the mechanism of nucleosome assembly. We show that yeast CAF-1 binding to a H3-H4 dimer activates the Cac1 winged helix domain interaction with DNA. This drives the formation of a transient CAF-1•histone•DNA intermediate containing two CAF-1 complexes, each associated with one H3-H4 dimer. Here, the (H3-H4)
Creator
Date Issued
  • 2017-03-18
Academic Affiliation
Journal Title
Journal Volume
  • 6
Subject
Last Modified
  • 2019-12-05
Identifier
  • PubMed ID: 28315523
Resource Type
Rights Statement
DOI
ISSN
  • 2050-084X
Language
License

Relationships

Items