Article
A Bulged Stem Tethers Est1p to Telomerase RNA in Budding Yeast Pubblico Deposited
https://scholar.colorado.edu/concern/articles/rx913q62j
- Abstract
- It is well established that the template for telomeric DNA synthesis is provided by the RNA subunit of telomerase; however, the additional functions provided by most of the rest of the RNA (>1000 nucleotides in budding yeast) are largely unknown. By alignment of telomerase RNAs of Saccharomyces cerevisiae and sixKluyveromycesspecies followed by mutagenesis of the S. cerevisiae RNA, we found a conserved region that is essential for telomere maintenance. Phylogenetic analysis and computer folding revealed that this region is conserved not only in primary nucleotide sequence but also in secondary structure. A common bulged-stem structure was predicted in all seven yeast species. Mutational analysis showed the structure to be essential for telomerase function. Suppression of bulged-stem mutant phenotypes by overexpression of Est1p and loss of co-immunoprecipitation of the mutant RNAs with Est1p indicated that this bulged stem is necessary for association of Est1p, a telomerase regulatory subunit. Est1p in yeast extract bound specifically to a small RNA containing the bulged stem, suggesting a direct interaction. We propose that this RNA structure links the enzymatic core of telomerase with Est1p, thereby allowing Est1p to recruit or activate telomerase at the telomere.
- Creator
- Date Issued
- 2002-11-01
- Academic Affiliation
- Journal Title
- Journal Volume
- 16
- File Extent
- 2800-2812
- Subject
- Ultima modifica
- 2019-12-05
- Resource Type
- Dichiarazione dei diritti
- DOI
- 10.1101/gad.1029302
- Language
Le relazioni
Elementi
Thumbnail | Titolo | Data caricata | Visibilità | Azioni |
---|---|---|---|---|
aBulgedStemTethersEst1PToTelomeraseRnaInBuddingYeast.pdf | 2019-12-05 | Pubblico | Scaricare |