Article

 

A map of protein dynamics during cell-cycle progression and cell-cycle exit. Público Deposited

https://scholar.colorado.edu/concern/articles/qr46r141q
Abstract
  • The cell-cycle field has identified the core regulators that drive the cell cycle, but we do not have a clear map of the dynamics of these regulators during cell-cycle progression versus cell-cycle exit. Here we use single-cell time-lapse microscopy of Cyclin-Dependent Kinase 2 (CDK2) activity followed by endpoint immunofluorescence and computational cell synchronization to determine the temporal dynamics of key cell-cycle proteins in asynchronously cycling human cells. We identify several unexpected patterns for core cell-cycle proteins in actively proliferating (CDK2-increasing) versus spontaneously quiescent (CDK2-low) cells, including Cyclin D1, the levels of which we find to be higher in spontaneously quiescent versus proliferating cells. We also identify proteins with concentrations that steadily increase or decrease the longer cells are in quiescence, suggesting the existence of a continuum of quiescence depths. Our single-cell measurements thus provide a rich resource for the field by characterizing protein dynamics during proliferation versus quiescence.
Creator
Date Issued
  • 2017-09-01
Academic Affiliation
Journal Title
Journal Issue/Number
  • 9
Journal Volume
  • 15
File Extent
  • 2003268-2003268
Subject
Publisher
Última modificação
  • 2019-12-05
Identifier
  • PubMed ID: 28892491
Resource Type
Declaração de direitos
DOI
ISSN
  • 1545-7885
Language
License

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