Article
The Apoptotic Engulfment Machinery Regulates Axonal Degeneration in C. elegans Neurons. Public Deposited
https://scholar.colorado.edu/concern/articles/pv63g093v
- Abstract
- Axonal degeneration is a characteristic feature of neurodegenerative disease and nerve injury. Here, we characterize axonal degeneration in Caenorhabditis elegans neurons following laser-induced axotomy. We show that this process proceeds independently of the WLD(S) and Nmnat pathway and requires the axonal clearance machinery that includes the conserved transmembrane receptor CED-1/Draper, the adaptor protein CED-6, the guanine nucleotide exchange factor complex Crk/Mbc/dCed-12 (CED-2/CED-5/CED-12), and the small GTPase Rac1 (CED-10). We demonstrate that CED-1 and CED-6 function non-cell autonomously in the surrounding hypodermis, which we show acts as the engulfing tissue for the severed axon. Moreover, we establish a function in this process for CED-7, an ATP-binding cassette (ABC) transporter, and NRF-5, a lipid-binding protein, both associated with release of lipid-vesicles during apoptotic cell clearance. Thus, our results reveal the existence of a WLD(S)/Nmnat-independent axonal degeneration pathway, conservation of the axonal clearance machinery, and a function for CED-7 and NRF-5 in this process.
- Creator
- Date Issued
- 2016-02-23
- Academic Affiliation
- Journal Title
- Journal Issue/Number
- 7
- Journal Volume
- 14
- File Extent
- 1673-1683
- Subject
- Nerve Degeneration
- Axotomy
- Cytoskeletal Proteins
- Apoptosis
- Animals
- Membrane Proteins
- rac GTP-Binding Proteins
- Proto-Oncogene Proteins c-crk
- ATP-Binding Cassette Transporters
- Caenorhabditis elegans
- Epidermis
- Neurons
- Phosphoproteins
- Gene Expression Regulation
- Carrier Proteins
- Signal Transduction
- Caenorhabditis elegans Proteins
- Last Modified
- 2019-12-05
- Identifier
- PubMed ID: 26876181
- Resource Type
- Rights Statement
- DOI
- ISSN
- 2211-1247
- Language
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