Article

 

Generation of a Mouse Full-length Balancer with Versatile Cassette-shuttling Selection Strategy. Public Deposited

https://scholar.colorado.edu/concern/articles/pk02cb292
Abstract
  • Balancer chromosomes are important tools for a variety of genetic manipulations in lower model organisms, owing to their ability to suppress recombination. In mouse, however, such effort has not been accomplished, mostly due to the size of the chromosomes and the complexity of multiple step chromosomal engineering. We developed an effective and versatile cassette-shuttling selection (CASS) strategy involving only two selection markers to achieve the sequential production of multiple large inversions along the chromosome. Using this strategy, we successfully generated the first full-length balancer in mice and showed that Balancer 17M-GFP can efficiently suppress recombination. Our study has not only generated a useful genetic resource, but also provided a strategy for constructing mammalian balancer chromosomes.
Creator
Date Issued
  • 2016-01-01
Academic Affiliation
Journal Title
Journal Issue/Number
  • 8
Journal Volume
  • 12
File Extent
  • 911-916
Subject
Dernière modification
  • 2019-12-05
Identifier
  • PubMed ID: 27489495
Resource Type
Déclaration de droits
DOI
ISSN
  • 1449-2288
Language

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