Article

 

Mechanistic Insights into Cofactor-Dependent Coupling of RNA Folding and mRNA Transcription/Translation by a Cobalamin Riboswitch. Public Deposited

https://scholar.colorado.edu/concern/articles/db78tc701
Abstract
  • Riboswitches are mRNA elements regulating gene expression in response to direct binding of a metabolite. While these RNAs are increasingly well understood with respect to interactions between receptor domains and their cognate effector molecules, little is known about the specific mechanistic relationship between metabolite binding and gene regulation by the downstream regulatory domain. Using a combination of cell-based, biochemical, and biophysical techniques, we reveal the specific RNA architectural features enabling a cobalamin-dependent hairpin loop docking interaction between receptor and regulatory domains. Furthermore, these data demonstrate that docking kinetics dictate a regulatory response involving the coupling of translation initiation to general mechanisms that control mRNA abundance. These results yield a comprehensive picture of how RNA structure in the riboswitch regulatory domain enables kinetically constrained ligand-dependent regulation of gene expression.
Creator
Date Issued
  • 2016-05-03
Academic Affiliation
Journal Title
Journal Issue/Number
  • 5
Journal Volume
  • 15
File Extent
  • 1100-1110
Subject
Dernière modification
  • 2019-12-05
Identifier
  • PubMed ID: 27117410
Resource Type
Déclaration de droits
DOI
ISSN
  • 2211-1247
Language

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