Title
Generation of a Mouse Full-length Balancer with Versatile Cassette-shuttling Selection Strategy.
Document Type
Article
Publication Date
1-1-2016
Publication Title
International Journal of Biological Sciences
ISSN
1449-2288
Volume
12
Issue
8
First Page
911
Last Page
916
DOI
https://doi.org/10.7150/ijbs.15172
PubMed ID
27489495
Abstract
Balancer chromosomes are important tools for a variety of genetic manipulations in lower model organisms, owing to their ability to suppress recombination. In mouse, however, such effort has not been accomplished, mostly due to the size of the chromosomes and the complexity of multiple step chromosomal engineering. We developed an effective and versatile cassette-shuttling selection (CASS) strategy involving only two selection markers to achieve the sequential production of multiple large inversions along the chromosome. Using this strategy, we successfully generated the first full-length balancer in mice and showed that Balancer 17M-GFP can efficiently suppress recombination. Our study has not only generated a useful genetic resource, but also provided a strategy for constructing mammalian balancer chromosomes.
Recommended Citation
Ye, Zhisheng; Sun, Lei; Li, Rongbo; Han, Min; Zhuang, Yuan; Wu, Xiaohui; and Xu, Tian, "Generation of a Mouse Full-length Balancer with Versatile Cassette-shuttling Selection Strategy." (2016). Molecular, Cellular, and Developmental Biology Faculty Contributions. 9.
https://scholar.colorado.edu/mcdb_facpapers/9