Document Type

Article

Publication Date

1-1-2016

Publication Title

International Journal of Biological Sciences

ISSN

1449-2288

Volume

12

Issue

8

First Page

911

Last Page

916

DOI

https://doi.org/10.7150/ijbs.15172

PubMed ID

27489495

Abstract

Balancer chromosomes are important tools for a variety of genetic manipulations in lower model organisms, owing to their ability to suppress recombination. In mouse, however, such effort has not been accomplished, mostly due to the size of the chromosomes and the complexity of multiple step chromosomal engineering. We developed an effective and versatile cassette-shuttling selection (CASS) strategy involving only two selection markers to achieve the sequential production of multiple large inversions along the chromosome. Using this strategy, we successfully generated the first full-length balancer in mice and showed that Balancer 17M-GFP can efficiently suppress recombination. Our study has not only generated a useful genetic resource, but also provided a strategy for constructing mammalian balancer chromosomes.

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