Date of Award
Spring 1-1-2016
Document Type
Dissertation
Degree Name
Doctor of Philosophy (PhD)
Department
Materials Science and Engineering
First Advisor
Jennifer N. Cha
Second Advisor
Joel L. Kaar
Third Advisor
Christopher N. Bowman
Fourth Advisor
Stephanie J. Bryant
Fifth Advisor
Daniel L. Feldheim
Abstract
Methods of high throughput, sensitive and cost effective quantification of proteins enables personalized medicine by allowing healthcare professionals to better monitor patient condition and response to treatment. My doctoral research has attempted to advance these methods through the use of filamentous bacteriophage (phage). These bacterial viruses are particularly amenable to both genetic and chemical engineering and can be produced efficiently in large amounts. Here, I discuss several strategies for modifying phage for use in protein sensing assays. These include the expression of bio-orthogonal conjugation handles on the phage coat, the incorporation of specific recognition sequences within the phage genome, and the creation of antibody-phage conjugates via a photo-crosslinking non-canonical amino acid. The physical and chemical characterization of these engineered phage and the results of their use in modified protein sensing assays will be presented.
Recommended Citation
Brasino, Michael David, "Engineering of Filamentous Bacteriophage for Protein Sensing" (2016). Materials Science & Engineering Graduate Theses & Dissertations. 4.
https://scholar.colorado.edu/mats_gradetds/4
Included in
Biochemistry Commons, Biomedical Engineering and Bioengineering Commons, Materials Science and Engineering Commons