Document Type

Article

Publication Date

9-3-2015

Publication Title

Nucleic acids research

ISSN

1362-4962

Volume

43

Issue

15

First Page

7535

Last Page

7543

DOI

http://dx.doi.org/10.1093/nar/gkv679

Abstract

FUS, a nuclear RNA-binding protein, plays multiple roles in RNA processing. Five specific FUS-binding RNA sequence/structure motifs have been proposed, but their affinities for FUS have not been directly compared. Here we find that human FUS binds all these sequences with Kd (app) values spanning a 10-fold range. Furthermore, some RNAs that do not contain any of these motifs bind FUS with similar affinity. FUS binds RNA in a length-dependent manner, consistent with a substantial non-specific component to binding. Finally, investigation of FUS binding to different nucleic acids shows that it binds single-stranded DNA with three-fold lower affinity than ssRNA of the same length and sequence, while binding to double-stranded nucleic acids is weaker. We conclude that FUS has quite general nucleic acid-binding activity, with the various proposed RNA motifs being neither necessary for FUS binding nor sufficient to explain its diverse binding partners.

Comments

Publication of this article was funded by the University of Colorado Boulder Libraries Open Access Fund.

This article was originally published in Nucleic Acids Research.

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