Type of Thesis
Molecular, Cellular, & Developmental Biology
Dr. Min Han
Dr. Jennifer Martin
Dr. Dylan Taatjes
Developmental robustness and redundancy are properties of biological systems that facilitate adaptation and survival in stressful environments. Through use of novel screens for synthetic phenotypes, we have elucidated a fascinating interaction between miRNAs and Vigilin, an RNA binding protein predicted to have a variety of different cellular functions within development. Synthetic screening indicates that mir-52, mir-59, mir-83, mir-254, and mir-265 all interact redundantly with Vigilin to modulate larval progression. Computational analysis and further RNAi testing yielded a list of potential downstream targets that these miRNAs might regulate to control development in a complex and robust manner. RT-qPCR and genetics will be used to study these potential targets to provide further insight into the interactions between miRNAs and Vigilin with the overarching goal of gaining a better understanding of development within C. elegans.
Weum, Brett, "The Interactions of Vigilin and the miRISC within Caenorhabditis elegans" (2015). Undergraduate Honors Theses. 945.