Type of Thesis
The ability to monitor cellular processes at high resolution using genetically encodable fluorescent protein tags and sensors has revolutionized life science research. In particular, several super-resolution imaging techniques take advantage of the phenomenon of stochastic fluorescent protein blinking. Here we present a method for measurement and analysis of the blinking behavior of red fluorescent proteins. This project contributes to the characterization of crucial photophysical parameters of fluorescent proteins used for super-resolution imaging techniques.
Simmerman, Emma, "Methods for photophysical characterization of red fluorescent proteins at the single-molecule level" (2019). Undergraduate Honors Theses. 1800.
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