Type of Thesis
Molecular, Cellular, & Developmental Biology
The endosomal sorting complexes required for transport (ESCRT) are protein complexes that remodel cellular membranes. The ESCRT III membrane machinery has been implicated in regulating membrane bound receptor proteins through the multi-vesicular body (MVB) pathway, during the end of cytokinesis, and being manipulated by the HIV virus during infection. My thesis project focuses on the in vivo regulation of ESCRT III. Bro1 binding to the Snf7 protein polymer helped stabilize ESCRT III. With this understanding that Bro 1 stabilizes the Snf7 polymer, two questions can be posed: Does it block Vps4 mediated Snf7 disassembly? Or does it block Vps2 and Vps24 from capping the Snf7 polymer? The latter of these two questions is the focus of my thesis. My research has shown the interaction of the capping Vps2:Vps24 dimer to only slightly affect protein sorting in the ESCRT pathway.
Coody, Troy K. III, "Stability of ESCRT III Snf7 Polymer Influenced by: Bro1 and Vps2/Vps24" (2016). Undergraduate Honors Theses. 1201.