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Minimally invasive multimode optical fiber microendoscope for deep brain fluorescence imaging. Public Deposited
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https://scholar.colorado.edu/concern/articles/c534fp68m
- Abstract
- A major open challenge in neuroscience is the ability to measure and perturb neural activity in vivo from well defined neural sub-populations at cellular resolution anywhere in the brain. However, limitations posed by scattering and absorption prohibit non-invasive multi-photon approaches for deep (>2mm) structures, while gradient refractive index (GRIN) endoscopes are relatively thick and can cause significant damage upon insertion. Here, we present a novel micro-endoscope design to image neural activity at arbitrary depths via an ultra-thin multi-mode optical fiber (MMF) probe that has 5-10X thinner diameter than commercially available micro-endoscopes. We demonstrate micron-scale resolution, multi-spectral and volumetric imaging. In contrast to previous approaches, we show that this method has an improved acquisition speed that is sufficient to capture rapid neuronal dynamics in-vivo in rodents expressing a genetically encoded calcium indicator (GCaMP). Our results emphasize the potential of this technology in neuroscience applications and open up possibilities for cellular resolution imaging in previously unreachable brain regions.
- Creator
- Date Issued
- 2018-04-01
- Academic Affiliation
- Journal Title
- Journal Issue/Number
- 4
- Journal Volume
- 9
- File Extent
- 1492-1509
- Last Modified
- 2019-12-05
- Identifier
- PubMed ID: 29675297
- Resource Type
- Rights Statement
- DOI
- ISSN
- 2156-7085
- Language
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