Article

 

Minimally invasive multimode optical fiber microendoscope for deep brain fluorescence imaging. Public Deposited

Downloadable Content

Download PDF
https://scholar.colorado.edu/concern/articles/c534fp68m
Abstract
  • A major open challenge in neuroscience is the ability to measure and perturb neural activity in vivo from well defined neural sub-populations at cellular resolution anywhere in the brain. However, limitations posed by scattering and absorption prohibit non-invasive multi-photon approaches for deep (>2mm) structures, while gradient refractive index (GRIN) endoscopes are relatively thick and can cause significant damage upon insertion. Here, we present a novel micro-endoscope design to image neural activity at arbitrary depths via an ultra-thin multi-mode optical fiber (MMF) probe that has 5-10X thinner diameter than commercially available micro-endoscopes. We demonstrate micron-scale resolution, multi-spectral and volumetric imaging. In contrast to previous approaches, we show that this method has an improved acquisition speed that is sufficient to capture rapid neuronal dynamics in-vivo in rodents expressing a genetically encoded calcium indicator (GCaMP). Our results emphasize the potential of this technology in neuroscience applications and open up possibilities for cellular resolution imaging in previously unreachable brain regions.
Creator
Date Issued
  • 2018-04-01
Academic Affiliation
Journal Title
Journal Issue/Number
  • 4
Journal Volume
  • 9
File Extent
  • 1492-1509
Last Modified
  • 2019-12-05
Identifier
  • PubMed ID: 29675297
Resource Type
Rights Statement
DOI
ISSN
  • 2156-7085
Language

Relationships

Items