Article
Achieving superresolution with illumination-enhanced sparsity. Public Deposited
Downloadable Content
Download PDF
https://scholar.colorado.edu/concern/articles/6d56zx34m
- Abstract
- Recent advances in superresolution fluorescence microscopy have been limited by a belief that surpassing two-fold resolution enhancement of the Rayleigh resolution limit requires stimulated emission or the fluorophore to undergo state transitions. Here we demonstrate a new superresolution method that requires only image acquisitions with a focused illumination spot and computational post-processing. The proposed method utilizes the focused illumination spot to effectively reduce the object size and enhance the object sparsity and consequently increases the resolution and accuracy through nonlinear image post-processing. This method clearly resolves 70nm resolution test objects emitting ~530nm light with a 1.4 numerical aperture (NA) objective, and, when imaging through a 0.5NA objective, exhibits high spatial frequencies comparable to a 1.4NA widefield image, both demonstrating a resolution enhancement above two-fold of the Rayleigh resolution limit. More importantly, we examine how the resolution increases with photon numbers, and show that the more-than-two-fold enhancement is achievable with realistic photon budgets.
- Creator
- Date Issued
- 2018-04-16
- Academic Affiliation
- Journal Title
- Journal Issue/Number
- 8
- Journal Volume
- 26
- File Extent
- 9850-9865
- Publisher
- Last Modified
- 2019-12-05
- Identifier
- PubMed ID: 29715931
- Resource Type
- Rights Statement
- DOI
- ISSN
- 1094-4087
- Language
Relationships
Items
Thumbnail | Title | Date Uploaded | Visibility | Actions |
---|---|---|---|---|
achievingSuperresolutionWithIlluminationEnhancedSparsity.pdf | 2019-12-05 | Public | Download |