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Achieving superresolution with illumination-enhanced sparsity. Public Deposited

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https://scholar.colorado.edu/concern/articles/6d56zx34m
Abstract
  • Recent advances in superresolution fluorescence microscopy have been limited by a belief that surpassing two-fold resolution enhancement of the Rayleigh resolution limit requires stimulated emission or the fluorophore to undergo state transitions. Here we demonstrate a new superresolution method that requires only image acquisitions with a focused illumination spot and computational post-processing. The proposed method utilizes the focused illumination spot to effectively reduce the object size and enhance the object sparsity and consequently increases the resolution and accuracy through nonlinear image post-processing. This method clearly resolves 70nm resolution test objects emitting ~530nm light with a 1.4 numerical aperture (NA) objective, and, when imaging through a 0.5NA objective, exhibits high spatial frequencies comparable to a 1.4NA widefield image, both demonstrating a resolution enhancement above two-fold of the Rayleigh resolution limit. More importantly, we examine how the resolution increases with photon numbers, and show that the more-than-two-fold enhancement is achievable with realistic photon budgets.
Creator
Date Issued
  • 2018-04-16
Academic Affiliation
Journal Title
Journal Issue/Number
  • 8
Journal Volume
  • 26
File Extent
  • 9850-9865
Publisher
Last Modified
  • 2019-12-05
Identifier
  • PubMed ID: 29715931
Resource Type
Rights Statement
DOI
ISSN
  • 1094-4087
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