Title

Compact diode laser source for multiphoton biological imaging

Document Type

Article

Publication Date

2017

Publication Title

Biomedical Optics Express

Volume

8

Issue

11

First Page

315

Last Page

322

DOI

https://doi.org/10.1364/BOE.8.000315

Abstract

We demonstrate a compact, pulsed diode laser source suitable for multiphoton microscopy of biological samples. The center wavelength is 976 nm, near the peak of the two-photon cross section of common fluorescent markers such as genetically encoded green and yellow fluorescent proteins. The laser repetition rate is electrically tunable between 66.67 kHz and 10 MHz, with 2.3 ps pulse duration and peak powers >1 kW. The laser components are fiber-coupled and scalable to a compact package. We demonstrate >600 μm depth penetration in brain tissue, limited by laser power.