Undergraduate Honors Thesis
The role of the Ou element in SL1 trans-splicing in C. elegans nematodes 公开 Deposited
https://scholar.colorado.edu/concern/undergraduate_honors_theses/mg74qm60n
- Abstract
- The nematode C. elegans exhibits SL1 trans-splicing, a unique form of pre-mRNA Processing seen in a variety of lower metazoans. In C. elegans, there are two types of trans-spliced leader that are added onto the 5' end of trans-spliced pre-mRNA: SL2 and its evolutionary predecessor, SL1. A bioinformatic analysis by Graber et al. (2007) revealed two conserved sequences associated with SL2 and SL1-accepting pre-mRNA known as the “Ur” and “Ou elements” respectively. Lasda et al. (2010) demonstrated that the Ur element is required for SL2 trans-splicing and predicted that the Ou element functions similarly for SL1 trans-splicing. The present study investigated the role of the Ou element in SL1 trans-splicing by gathering primary data using mutational analysis followed by SL1 in vitro trans-splicing assays. Two different Ou-mutations considerably decreased SL1 trans-splicing efficiency when compared to wild-type constructs of the same gene. These data suggest that the Ou element may play a key role in SL1 trans-splicing.
- Creator
- Date Awarded
- 2011-10-25
- Academic Affiliation
- Advisor
- Granting Institution
- Subject
- 最新修改
- 2019-12-02
- Resource Type
- 权利声明
- Language
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缩略图 | 标题 | 上传日期 | 公开度 | 行动 |
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theRoleOfTheOuElementInSl1TransSplicingInCElegansNe.pdf | 2019-11-19 | 公开 | 下载 |