Undergraduate Honors Thesis

 

The role of the Ou element in SL1 trans-splicing in C. elegans nematodes Público Deposited

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https://scholar.colorado.edu/concern/undergraduate_honors_theses/mg74qm60n
Abstract
  • The nematode C. elegans exhibits SL1 trans-splicing, a unique form of pre-mRNA Processing seen in a variety of lower metazoans. In C. elegans, there are two types of trans-spliced leader that are added onto the 5' end of trans-spliced pre-mRNA: SL2 and its evolutionary predecessor, SL1. A bioinformatic analysis by Graber et al. (2007) revealed two conserved sequences associated with SL2 and SL1-accepting pre-mRNA known as the “Ur” and “Ou elements” respectively. Lasda et al. (2010) demonstrated that the Ur element is required for SL2 trans-splicing and predicted that the Ou element functions similarly for SL1 trans-splicing. The present study investigated the role of the Ou element in SL1 trans-splicing by gathering primary data using mutational analysis followed by SL1 in vitro trans-splicing assays. Two different Ou-mutations considerably decreased SL1 trans-splicing efficiency when compared to wild-type constructs of the same gene. These data suggest that the Ou element may play a key role in SL1 trans-splicing.
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  • 2011-10-25
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  • 2019-12-02
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