Identification of Sfi1 protein interactions through yeast two-hybrid analysis

Undergraduate Honors Thesis

Citations

Citeable URL: https://scholar.colorado.edu/concern/undergraduate_honors_theses/bg257f534

Abstract

The most notable eukaryotic microtubule organizing center, the centrosome, forms the mitotic spindle required for mitosis and meiosis3. Problems with centrosomal duplication and function can lead to chromosomal missegregation, which has been linked to numerous human diseases and cancer3. Though the structure has been closely studied in different organisms, the mechanism by which it duplicates is still not well understood5. In the budding yeast Saccharomyces cerevisiae the spindle pole body is analogous to the human centrosome, and has numerous highly conserved components, making it an excellent model to study to better understand centrosome duplication and function5. This study aimed to identify protein interactions of Sfi1, one essential and highly conserved SPB component, in order to characterize its role in SPB duplication. Through a yeast two-hybrid screen, the C terminus of Sfi1 was found to interact with RNA II polymerase mediator complex protein Cse2. This interaction provides more evidence for Cse2 being involved at the spindle, and further analysis of the interaction will elucidate the role of Sfi1 in SPB duplication.

Creator