Graduate Thesis Or Dissertation
Engineering of Filamentous Bacteriophage for Protein Sensing Public Deposited
https://scholar.colorado.edu/concern/graduate_thesis_or_dissertations/h702q660k
- Abstract
- Methods of high throughput, sensitive and cost effective quantification of proteins enables personalized medicine by allowing healthcare professionals to better monitor patient condition and response to treatment. My doctoral research has attempted to advance these methods through the use of filamentous bacteriophage (phage). These bacterial viruses are particularly amenable to both genetic and chemical engineering and can be produced efficiently in large amounts. Here, I discuss several strategies for modifying phage for use in protein sensing assays. These include the expression of bio-orthogonal conjugation handles on the phage coat, the incorporation of specific recognition sequences within the phage genome, and the creation of antibody-phage conjugates via a photo-crosslinking non-canonical amino acid. The physical and chemical characterization of these engineered phage and the results of their use in modified protein sensing assays will be presented.
- Creator
- Date Issued
- 2016
- Academic Affiliation
- Advisor
- Committee Member
- Degree Grantor
- Commencement Year
- Subject
- Dernière modification
- 2019-11-14
- Resource Type
- Déclaration de droits
- Language
Des relations
Articles
La vignette | Titre | Date de téléchargement | Visibilité | actes |
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engineeringOfFilamentousBacteriophageForProteinSensing.pdf | 2019-11-14 | Public | Télécharger |