Graduate Thesis Or Dissertation

 

Monoclonal Antibody Aggregation in Cell Culture: Aggregate Characterization and Protein Stability Analysis Public Deposited

https://scholar.colorado.edu/concern/graduate_thesis_or_dissertations/0r967385d
Abstract
  • Protein aggregates represent a safety, immunological and stability concern in therapeutic protein formulations. Aggregates may be formed at any stage during the manufacturing process, including during cell culture. In mammalian cell culture, the proteins that are produced typically are incubated in the cell culture medium at relatively high temperatures (e.g. 36 ºC) for prolonged periods of time (1-2 weeks). To investigate potential mechanisms by which this incubation might generate protein aggregates, we examined the stability of a model monoclonal antibody (mAb) under cell culture conditions, but in the absence of cells. A step-wise approach was used to eliminate factors that would be causing protein aggregation. Results show that protein aggregation is mediated by the presence of extracellular monoclonal antibody light chains. This result was corroborated through aggregate characterization showing that a cell lines aggregation propensity correlated with the light chain content of HMWS.
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  • 2015
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  • 2019-11-14
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