Graduate Thesis Or Dissertation

 

Synthesis of Phosphonoacetate RNA and a Two-Step RNA Synthesis Pubblico Deposited

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https://scholar.colorado.edu/concern/graduate_thesis_or_dissertations/02870v98g
Abstract
  • Phosphonoacetate RNA dimers and oligomers were synthesized using solid phase synthesis. Two different, orthoganol approaches were used for the 2’ and 5’ oxygens of the phosphoramidite synthons in order to maintain the phosphonoacetate linkage upon 2’-O deprotection. The first utilized a 2’-O-silyl protection (fluoride labile) along with a 5’-O-DMT protection (acid labile). The second path used an inverse 2’-O-ACE (acid labile) protecting group along with a 5’-O-silyl protection strategy. An additional emphasis in this dissertation was on the solid phase synthesis of unmodified RNA using a two-step cycle. The synthesis was performed using a 5’-O-carbonate-2’-O-1,3-Benzodithiolylium (BDT) protected synthon. During synthesis a peroxy anion buffer removes the 5’-carbonate protection and simultaneously oxidizes the internucleotide phosphite linkage. Following synthesis and removal from support the 2’-O-BDT group was removed. All products were then isolated and characterized.
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  • 2013-01-01
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  • 2019-11-13
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