Graduate Thesis Or Dissertation
Synthesis of Phosphonoacetate RNA and a Two-Step RNA Synthesis Public Deposited
Contenu téléchargeable
Télécharger le fichier PDF
https://scholar.colorado.edu/concern/graduate_thesis_or_dissertations/02870v98g
- Abstract
- Phosphonoacetate RNA dimers and oligomers were synthesized using solid phase synthesis. Two different, orthoganol approaches were used for the 2’ and 5’ oxygens of the phosphoramidite synthons in order to maintain the phosphonoacetate linkage upon 2’-O deprotection. The first utilized a 2’-O-silyl protection (fluoride labile) along with a 5’-O-DMT protection (acid labile). The second path used an inverse 2’-O-ACE (acid labile) protecting group along with a 5’-O-silyl protection strategy. An additional emphasis in this dissertation was on the solid phase synthesis of unmodified RNA using a two-step cycle. The synthesis was performed using a 5’-O-carbonate-2’-O-1,3-Benzodithiolylium (BDT) protected synthon. During synthesis a peroxy anion buffer removes the 5’-carbonate protection and simultaneously oxidizes the internucleotide phosphite linkage. Following synthesis and removal from support the 2’-O-BDT group was removed. All products were then isolated and characterized.
- Creator
- Date Issued
- 2013-01-01
- Academic Affiliation
- Advisor
- Committee Member
- Degree Grantor
- Commencement Year
- Subject
- Dernière modification
- 2019-11-13
- Resource Type
- Déclaration de droits
- Language
Des relations
Articles
La vignette | Titre | Date de téléchargement | Visibilité | actes |
---|---|---|---|---|
synthesisOfPhosphonoacetateRnaAndATwoStepRnaSynthesis.pdf | 2019-11-13 | Public | Télécharger |