Graduate Thesis Or Dissertation

 

Modeling Cytoskeletal Active Matter Systems Public Deposited

https://scholar.colorado.edu/concern/graduate_thesis_or_dissertations/02870v844
Abstract
  • Active networks of filamentous proteins and crosslinking motor proteins play a critical role in many important cellular processes. One of the most important microtubule-motor protein assemblies is the mitotic spindle, a self-organized active liquid-crystalline structure that forms during cell division and that ultimately separates chromosomes into two daughter cells. Although the spindle has been intensively studied for decades, the physical principles that govern its self-organization and function remain mysterious. To evolve a better understanding of spindle formation, structure, and dynamics, I investigate course-grained models of active liquid-crystalline networks composed of microtubules, modeled as hard spherocylinders, in diffusive equilibrium with a reservoir of active crosslinks, modeled as hookean springs that can adsorb to microtubules and and translocate at finite velocity along the microtubule axis. This model is investigated using a combination of brownian dynamics and kinetic monte carlo simulation. I have further refined this model to simulate spindle formation and kinetochore capture in the fission yeast S. pombe. I then make predictions for experimentally realizable perturbations in motor protein presence and function in S. pombe.
Creator
Date Issued
  • 2016
Academic Affiliation
Advisor
Committee Member
Degree Grantor
Commencement Year
Subject
Last Modified
  • 2019-11-15
Resource Type
Rights Statement
Language

Relationships

Items