Article

Abstract

• Drosophila melanogaster larvae irradiated with doses of ionizing radiation (IR) that kill about half of the cells in larval imaginal discs still develop into viable adults. How surviving cells compensate for IR-induced cell death to produce organs of normal size and appearance remains an active area of investigation. We have identified a subpopulation of cells within the continuous epithelium of Drosophila larval wing discs that shows intrinsic resistance to IR- and drug-induced apoptosis. These cells reside in domains of high Wingless (Wg, Drosophila Wnt-1) and STAT92E (sole Drosophila signal transducer and activator of transcription [STAT] homolog) activity and would normally form the hinge in the adult fly. Resistance to IR-induced apoptosis requires STAT and Wg and is mediated by transcriptional repression of the pro-apoptotic gene reaper. Lineage tracing experiments show that, following irradiation, apoptosis-resistant cells lose their identity and translocate to areas of the wing disc that suffered abundant cell death. Our findings provide a new paradigm for regeneration in which it is unnecessary to invoke special damage-resistant cell types such as stem cells. Instead, differences in gene expression within a population of genetically identical epithelial cells can create a subpopulation with greater resistance, which, following damage, survive, alter their fate, and help regenerate the tissue.

Creator

• Verghese, Shilpi
• Su, Tin Tin

Date Issued

• 2016-09-01

Academic Affiliation

• Molecular, Cellular & Developmental Biology (MCDB)

Journal Title

• PLoS Biology

Journal Issue/Number

• 9

Journal Volume

• 14

File Extent

• 1002536-1002536

Subject

• Drosophila melanogaster
• Gene Expression
• Experimental
• Apoptosis
• Imaginal Discs
• Regeneration
• Larva
• Wnt1 Protein
• Gene Silencing
• Radiation Injuries
• Drosophila Proteins
• Animals
• Epithelial Cells
• STAT Transcription Factors

Publisher

• Public Library of Science

Ultima modifica

• 2019-12-05

Identifier

• PubMed ID: 27584613

Resource Type

• Article

Dichiarazione dei diritti

• In Copyright

DOI

•  https://doi.org/10.1371/journal.pbio.1002536

ISSN

• 1545-7885

Language

• English [eng]

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