Article

 

Visualizing the metazoan proliferation-quiescence decision in vivo Public Deposited

https://scholar.colorado.edu/concern/articles/zc77sr394
Abstract
  • Cell proliferation and quiescence are intimately coordinated during metazoan development. Here, we adapt a cyclin-dependent kinase (CDK) sensor to uncouple these key events of the cell cycle in Caenorhabditis elegans and zebrafish through live-cell imaging. The CDK sensor consists of a fluorescently tagged CDK substrate that steadily translocates from the nucleus to the cytoplasm in response to increasing CDK activity and consequent sensor phosphorylation. We show that the CDK sensor can distinguish cycling cells in G1 from quiescent cells in G0, revealing a possible commitment point and a cryptic stochasticity in an otherwise invariant C. elegans cell lineage. Finally, we derive a predictive model of future proliferation behavior in C. elegans based on a snapshot of CDK activity in newly born cells. Thus, we introduce a live-cell imaging tool to facilitate in vivo studies of cell-cycle control in a wide-range of developmental contexts.

Creator
Date Issued
  • 2020
Academic Affiliation
Journal Title
Journal Volume
  • 9
Last Modified
  • 2022-05-19
Resource Type
Rights Statement
DOI
ISSN
  • 2050-084X
Language
License

Relationships

Items