Article

 

Characterizing and correcting camera noise in back-illuminated sCMOS cameras Public Deposited

https://scholar.colorado.edu/concern/articles/qv33rz100
Abstract
  • With promising properties of fast imaging speed, large field-of-view, relative low cost and many others, back-illuminated sCMOS cameras have been receiving intensive attention for low light level imaging in the past several years. However, due to the pixel-to-pixel difference of camera noise (called noise non-uniformity) in sCMOS cameras, researchers may hesitate to use them in some application fields, and sometimes wonder whether they should optimize the noise non-uniformity of their sCMOS cameras before using them in a specific application scenario. In this paper, we systematically characterize the impact of different types of sCMOS noise on image quality and perform corrections to these types of sCMOS noise using three representative algorithms (PURE, NCS and MLEsCMOS). We verify that it is possible to use appropriate correction methods to push the non-uniformity of major types of camera noise, including readout noise, offset, and photon response, to a satisfactory level for conventional microscopy and single molecule localization microscopy. We further find out that, after these corrections, global read noise becomes a major concern that limits the imaging performance of back-illuminated sCMOS cameras. We believe this study provides new insights into the understanding of camera noise in back-illuminated sCMOS cameras, and also provides useful information for future development of this promising camera technology.

     

Creator
Date Issued
  • 2021
Academic Affiliation
Journal Title
Journal Issue/Number
  • 5
Journal Volume
  • 21
Last Modified
  • 2022-08-18
Resource Type
Rights Statement
DOI
ISSN
  • 1094-4087
Language

Relationships

Items