Article

 

Live Cell Imaging Reveals pH Oscillations in Saccharomyces cerevisiae During Metabolic Transitions. Öffentlichkeit Deposited

https://scholar.colorado.edu/concern/articles/h128nf20b
Abstract
  • Addition of glucose to starved Saccharomyces cerevisiae initiates collective NADH dynamics termed glycolytic oscillations. Numerous questions remain about the extent to which single cells can oscillate, if oscillations occur in natural conditions, and potential physiological consequences of oscillations. In this paper, we report sustained glycolytic oscillations in single cells without the need for cyanide. Glucose addition to immobilized cells induced pH oscillations that could be imaged with fluorescent sensors. A population of cells had oscillations that were heterogeneous in frequency, start time, stop time, duration and amplitude. These changes in cytoplasmic pH were necessary and sufficient to drive changes in NADH. Oscillators had lower mitochondrial membrane potentials and budded more slowly than non-oscillators. We also uncovered a new type of oscillation during recovery from H
Creator
Date Issued
  • 2017-10-24
Academic Affiliation
Journal Title
Journal Issue/Number
  • 1
Journal Volume
  • 7
File Extent
  • 13922-13922
Zuletzt geändert
  • 2019-12-05
Identifier
  • PubMed ID: 29066766
Resource Type
Urheberrechts-Erklärung
DOI
ISSN
  • 2045-2322
Language
License

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