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Ki67 is a Graded Rather than a Binary Marker of Proliferation versus Quiescence. Public Deposited

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https://scholar.colorado.edu/concern/articles/gt54kn77p
Abstract
  • Ki67 staining is widely used as a proliferation indicator in the clinic, despite poor understanding of this protein's function or dynamics. Here, we track Ki67 levels under endogenous control in single cells over time and find that Ki67 accumulation occurs only during S, G2, and M phases. Ki67 is degraded continuously in G1 and G0 phases, regardless of the cause of entry into G0/quiescence. Consequently, the level of Ki67 during G0 and G1 in individual cells is highly heterogeneous and depends on how long an individual cell has spent in G0. Thus, Ki67 is a graded rather than a binary marker both for cell-cycle progression and time since entry into quiescence.
Creator
Date Issued
  • 2018-07-31
Academic Affiliation
Journal Title
Journal Issue/Number
  • 5
Journal Volume
  • 24
File Extent
  • 1105-1112
Last Modified
  • 2019-12-05
Identifier
  • PubMed ID: 30067968
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DOI
ISSN
  • 2211-1247
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