Article

Abstract

• Ki67 staining is widely used as a proliferation indicator in the clinic, despite poor understanding of this protein's function or dynamics. Here, we track Ki67 levels under endogenous control in single cells over time and find that Ki67 accumulation occurs only during S, G2, and M phases. Ki67 is degraded continuously in G1 and G0 phases, regardless of the cause of entry into G0/quiescence. Consequently, the level of Ki67 during G0 and G1 in individual cells is highly heterogeneous and depends on how long an individual cell has spent in G0. Thus, Ki67 is a graded rather than a binary marker both for cell-cycle progression and time since entry into quiescence.

Creator

• Tian, Chengzhe
• Miller, Iain
• Yang, Chen
• Carter, Dylan
• Spencer, Sabrina L
• Gookin, Sara
• Min, Mingwei

Date Issued

• 2018-07-31

Academic Affiliation

• Biochemistry

Journal Title

• Cell Reports

Journal Issue/Number

• 5

Journal Volume

• 24

File Extent

• 1105-1112

Last Modified

• 2019-12-05

Identifier

• PubMed ID: 30067968

Resource Type

• Article

Rights Statement

• In Copyright

DOI

•  https://doi.org/10.1016/j.celrep.2018.06.110

ISSN

• 2211-1247

Language

• English [eng]

License

• Creative Commons BY-NC-ND Attribution-NonCommercial-NoDerivs 4.0 International

Relationships

Items

Thumbnail	Title	Date Uploaded	Visibility	Actions
	ki67IsAGradedRatherThanABinaryMarkerOfProliferationVer.pdf	2019-12-05	Public	Download