Article

 

Circular RNAs Co-Precipitate with Extracellular Vesicles: A Possible Mechanism for circRNA Clearance. Public Deposited

Downloadable Content

Download PDF
https://scholar.colorado.edu/concern/articles/fn106z604
Abstract
  • Backspliced circular RNAs (circRNAs) are prevalent in many eukaryotic systems and are spliced from thousands of different genes. Where examined, circRNAs are often highly stable and the mechanisms by which cells degrade and/or clear circRNAs from the cells are unknown. Here we investigated the possibility that cells can eliminate circRNAs into extracellular space, possibly within released vesicles such as exosomes and microvesicles. From three different cell lines and examining multiple circRNAs, we show that extracellular vesicle (EVs) preparations recovered from cell culture conditioned media contain established circRNAs. Moreover, these circRNAs are enriched over their linear counterparts within EV preparations when compared to the producing cells. This supports the idea that expulsion from cells into extracellular space, as by EVs release, can be a mechanism by which cells clear circRNAs. Moreover, since EVs can be taken up by other cells, excreted circRNAs could contribute to cell to cell communication.
Creator
Date Issued
  • 2016-01-01
Academic Affiliation
Journal Title
Journal Issue/Number
  • 2
Journal Volume
  • 11
File Extent
  • 0148407-0148407
Subject
Last Modified
  • 2019-12-05
Identifier
  • PubMed ID: 26848835
Resource Type
Rights Statement
DOI
ISSN
  • 1932-6203
Language

Relationships

Items