Article
Reducing Tumour Hypoxia via Oral Administration of Oxygen Nanobubbles. Pubblico Deposited
https://scholar.colorado.edu/concern/articles/bg257f819
- Abstract
- Hypoxia has been shown to be a key factor inhibiting the successful treatment of solid tumours. Existing strategies for reducing hypoxia, however, have shown limited efficacy and/or adverse side effects. The aim of this study was to investigate the potential for reducing tumour hypoxia using an orally delivered suspension of surfactant-stabilised oxygen nanobubbles. Experiments were carried out in a mouse xenograft tumour model for human pancreatic cancer (BxPc-3 cells in male SCID mice). A single dose of 100 μL of oxygen saturated water, oxygen nanobubbles or argon nanobubbles was administered via gavage. Animals were sacrificed 30 minutes post-treatment (3 per group) and expression of hypoxia-inducible-factor-1α (HIF1α) protein measured by real time quantitative polymerase chain reaction and Western blot analysis of the excised tumour tissue. Neither the oxygen saturated water nor argon nanobubbles produced a statistically significant change in HIF1α expression at the transcriptional level. In contrast, a reduction of 75% and 25% in the transcriptional and translational expression of HIF1α respectively (p
- Creator
- Date Issued
- 2016-01-01
- Academic Affiliation
- Journal Title
- Journal Issue/Number
- 12
- Journal Volume
- 11
- File Extent
- 0168088-0168088
- Subject
- alpha Subunit
- Signal Transduction
- Male
- Pancreatic Neoplasms
- Disease Models
- Humans
- Vascular Endothelial Growth Factor A
- Xenograft Model Antitumor Assays
- Neovascularization
- Mice
- Hypoxia-Inducible Factor 1
- Nanoparticles
- Oral
- Animal
- Nanostructures
- Pathologic
- Angiogenesis Inhibitors
- Oxygen
- Tumor Hypoxia
- Administration
- SCID
- Cell Line
- Tumor
- Gene Expression Regulation
- Animals
- Neoplastic
- Ultima modifica
- 2019-12-06
- Identifier
- PubMed ID: 28036332
- Resource Type
- Dichiarazione dei diritti
- DOI
- ISSN
- 1932-6203
- Language
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