Graduate Thesis Or Dissertation
Automated Analysis Pipeline for Inferring Phenotypic Changes in Cell Granules Public Deposited
https://scholar.colorado.edu/concern/graduate_thesis_or_dissertations/t148fh57w
- Abstract
- In Saccharomyces cerevisiae, P-bodies play critical roles in the control of gene expression in localized cellular domains. Many P-bodies components contain intrinsically disordered regions (IDR). This thesis explores the hypothesis that the IDR of one P-body component Dhh1 plays a role in P-body assembly. To answer this question, an automated analysis pipeline was created to compare changes in the phenotypes of P-bodies in the full length Dhh1 protein and the Dhh1 protein without the IDR (d_IDR). I found that the IDR of Dhh1, though not necessary for Dhh1 recruitment to P-bodies, increases the number of P-bodies in each cell and decreases the relative concentration of P-bodies constituents. The IDR of Dhh1 was also found not to have an effect on P-bodies size. The results of these analyses support the original hypothesis that the IDR of Dhh1 assists in P-body assembly through promiscuous and synergistic interactions with other proteins found within the P-body. The automated analysis pipeline that I created was found to be accurate when compared to existing manual analysis methods and could be generalized to analyze the phenotypes of other cellular granules.
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- Date Issued
- 2017
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- Last Modified
- 2019-11-18
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Thumbnail | Title | Date Uploaded | Visibility | Actions |
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automatedAnalysisPipelineForInferringPhenotypicChangesInC.pdf | 2019-11-18 | Public | Download |