Article

Abstract

• Polycomb repressive complex 2 (PRC2) is a key chromatin modifier responsible for methylation of lysine 27 in histone H3. PRC2 has been shown to interact with thousands of RNA species in vivo, but understanding the physiological function of RNA binding has been hampered by the lack of separation-of-function mutants. Here, we use comprehensive mutagenesis and hydrogen deuterium exchange mass spectrometry (HDX-MS) to identify critical residues for RNA interaction in PRC2 core complexes from

Creator

• Goodrich, Karen J
• Long, Yicheng
• Chen, Siming
• Maegley, Karen A
• Gong, Lihu
• Liu, Wei
• Brooun, Alexei
• Gooding, Anne R
• Cech, Thomas R
• Yang, Xin
• Gajiwala, Ketan S
• Bolanos, Ben
• Liu, Xin

Date Issued

• 2017-11-29

Academic Affiliation

• Chemical and Biological Engineering

Journal Title

• Elife

Journal Volume

• 6

Subject

• G-quadruplex RNA
• human
• PRC2
• Chaetomium thermophilum
• HDX-MS
• biochemistry
• RNA binding proteins
• epigenetics

Last Modified

• 2019-12-05

Identifier

• PubMed ID: 29185984

Resource Type

• Article

Rights Statement

• In Copyright

DOI

•  https://doi.org/10.7554/eLife.31558

ISSN

• 2050-084X

Language

• English [eng]

License

• Creative Commons BY Attribution 4.0 International

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