Molecular Systems Biology
Our limited ability to predict genotype-phenotype relationships has called for strategies that allow testing of thousands of hypotheses in parallel. Deep scanning mutagenesis has been successfully implemented to map genotype-phenotype relationships at a single-protein scale, allowing scientists to elucidate properties that are difficult to predict. However, most phenotypes are dictated by several proteins that are interconnected through complex and robust regulatory and metabolic networks. These sophisticated networks hinder our understanding of the phenotype of interest and limit our capabilities to rewire cellular functions. Here, we leveraged CRISPR-EnAbled Trackable genome Engineering to attempt a parallel and high-resolution interrogation of complex networks, deep scanning multiple proteins associated with lysine metabolism in
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Bassalo, Marcelo C; Garst, Andrew D; Choudhury, Alaksh; Grau, William C; Oh, Eun J; Spindler, Eileen; Lipscomb, Tanya; and Gill, Ryan T, "Deep scanning lysine metabolism in Escherichia coli" (2018). Chemical & Biological Engineering Faculty Contributions. 6.