Article
Nucleic acid-binding specificity of human FUS protein. Public Deposited
Downloadable Content
Download PDF- Abstract
FUS, a nuclear RNA-binding protein, plays multiple roles in RNA processing. Five specific FUS-binding RNA sequence/structure motifs have been proposed, but their affinities for FUS have not been directly compared. Here we find that human FUS binds all these sequences with Kd (app) values spanning a 10-fold range. Furthermore, some RNAs that do not contain any of these motifs bind FUS with similar affinity. FUS binds RNA in a length-dependent manner, consistent with a substantial non-specific component to binding. Finally, investigation of FUS binding to different nucleic acids shows that it binds single-stranded DNA with three-fold lower affinity than ssRNA of the same length and sequence, while binding to double-stranded nucleic acids is weaker. We conclude that FUS has quite general nucleic acid-binding activity, with the various proposed RNA motifs being neither necessary for FUS binding nor sufficient to explain its diverse binding partners.
- Creator
- Date Issued
- 2015-09-03
- Academic Affiliation
- Journal Title
- Journal Issue/Number
- 15
- Journal Volume
- 43
- File Extent
- 7535-7543
- Subject
- Publisher
- Last Modified
- 2020-01-09
- Identifier
- PubMed ID: 26150427
- Resource Type
- Rights Statement
- DOI
- ISSN
- 1362-4962
- Language
Relationships
- In Collection:
Items
Thumbnail | Title | Date Uploaded | Visibility | Actions |
---|---|---|---|---|
nucleicAcidBindingSpecificityOfHumanFusProtein.pdf | 2019-12-09 | Public | Download | |
gkv679SupplementaryData.zip | 2019-12-09 | Public | Download |