Undergraduate Honors Thesis

 

The Interactions of Vigilin and the miRISC within Caenorhabditis elegans Public Deposited

https://scholar.colorado.edu/concern/undergraduate_honors_theses/ks65hc66f
Abstract
  • Developmental robustness and redundancy are properties of biological systems that facilitate adaptation and survival in stressful environments. Through use of novel screens for synthetic phenotypes, we have elucidated a fascinating interaction between miRNAs and Vigilin, an RNA binding protein predicted to have a variety of different cellular functions within development. Synthetic screening indicates that mir-52, mir-59, mir-83, mir-254, and mir-265 all interact redundantly with Vigilin to modulate larval progression. Computational analysis and further RNAi testing yielded a list of potential downstream targets that these miRNAs might regulate to control development in a complex and robust manner. RT-qPCR and genetics will be used to study these potential targets to provide further insight into the interactions between miRNAs and Vigilin with the overarching goal of gaining a better understanding of development within C. elegans.
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  • 2015-01-01
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  • 2019-12-02
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