Graduate Thesis Or Dissertation

 

Recognition of Outer Membrane Proteins by the β-Barrel Assembly Machine in Escherichia Coli Public Deposited

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https://scholar.colorado.edu/concern/graduate_thesis_or_dissertations/sx61dm73q
Abstract
  • In E. coli, the β-barrel Assembly Machinery (BAM) is an outer membrane protein complex which catalyzes the folding and insertion of integral outer membrane proteins. This thesis explores the hypothesis that BAM recognizes substrates by a sequence specific motif. A high-throughput screening approach called the SPOT assay is used to determine if BAM subunits bind to previously implicated sequence motifs or some yet unidentified consensus sequence. For this assay, solutions of fluorescently labeled BAM subunits are used to probe peptide arrays displaying sequences derived from BAM substrates, and binding is visualized by increased fluorescence. Results of these experiments do not support the original hypothesis of sequence-specific recognition by BAM, and we propose that BAM may recognize substrates in a general fashion that does not require a targeting sequence.
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  • 2016
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  • 2019-11-18
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