Date of Award

Spring 1-1-2014

Document Type

Dissertation

Degree Name

Doctor of Philosophy (PhD)

Department

Chemistry & Biochemistry

First Advisor

Amy E. Palmer

Second Advisor

Natalie G. Ahn

Third Advisor

Gia K. Voeltz

Fourth Advisor

Xuedong Liu

Fifth Advisor

Greg Odorizzi

Abstract

Salmonella species are Gram-negative bacteria responsible for causing a variety of diseases in multiple hosts across the plant and animal kingdom. In humans, Salmonella are most known for causing typhoid fever and gastroenteritis. In both of these diseases, Salmonella use effector proteins to generate a niche within host cells where the bacteria can survive and/or replicate. The niche, which is composed of a membrane-bound vacuole called the Salmonella containing vacuole or SCV, is trafficked and matured throughout infection. This process requires the work of both effector proteins and host cell proteins, which are recruited to the SCV. Although the sequence of events behind SCV formation and maturation is known, the mechanism behind SCV development is poorly understood. Here we discuss two novel imaging methods and subsequent quantitative analysis techniques we created to study the roles of the specific effector proteins SteA and SseG in the proper establishment and maturation of the SCV during infection of single cells in both gastroenteritis and typhoid fever models.

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